|
KMID : 0545120080180040686
|
|
Journal of Microbiology and Biotechnology
2008 Volume.18 No. 4 p.686 ~ p.694
|
|
|
Effects of a Tetramethoxyhydroxyflavone p7F on the Expression of Inflammatory Mediators in LPS-treated Human Synovial Fibroblast and Macrophage Cells
|
|
Yoon Do-Young
Cho Min-Chul
Kim Jung-Hee
Kim Eun-Jin
Kang Jeong-Woo
Seo Eun-Hee
Shim Jung-Hyun
Kim Soo-Hyun
Lee Hee-Gu
Oh Goo-Taeg
Hong Jin-Tae
Park Joo-Won
Kim Jong-Wan
|
|
|
Abstract
|
|
|
|
The inhibitory effects of 5,6,3’,5’-tetramethoxy 7,4’-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1β or TNF-α induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin E2 in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1β and thus proliferates), revealing that p7F inhibited IL-1β-induced proliferation of D10S Th2 cells in a doseresponse manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IκB degradation and NF-κB activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an antiinflammatory agent for inhibiting inflammatory responses.
|
|
|
KEYWORD
|
|
|
Anti-inflammatory agent, flavone, synovial fibroblasts, inflammatory mediators, proinflammatory cytokines
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|